Plasmid DNA Production

Plasmids are widely used in biotechnology as a primary substrate for protein production. In this instance plasmids serve as vectors for introducing the desired gene into host cells. This enables production of large quantities of recombinant proteins. These circular strands of DNA can be optimized to ensure efficient expression and high production yields for the target protein, making them invaluable tools in research and biopharmaceutical applications.

It is critical that plasmid solutions used for protein production contain only low levels of endotoxin contaminants. High endotoxin levels can trigger immune responses and inhibit proper protein folding, which ultimately compromises end-product quality. Additionally, maintaining a high plasmid supercoil level is essential for robust protein expression, as it enhances transcriptional efficiency and the stability of the gene, leading to increased yields of the desired protein. The combination of low endotoxin levels and high supercoil levels ensures safer and more efficient protein production processes that result in higher yields of biologically active and pure proteins.

At PackGene, we take pride in our antibiotic-free fermentation process and 3-step purification methods. Combining these rigorous protocols guarantees a plasmid with exceptionally high supercoiling and remarkably low endotoxin levels. Our commitment to stringent quality control and management ensures that downstream protein expression consistently reaches optimal levels, providing our customers with biologically active and pure proteins for their diverse applications.

Experience the gold standard in AAV plasmid production at PackGene! We understand the complexities that come with the inclusion of Inverted Terminal Repeat (ITR) sequences that are required in AAV plasmid functionality. We have processes in place to ensure high-fidelity AAV plasmid production in the face of complications caused by ITR inclusion.

From the earliest plasmid production stage, we take meticulous steps to verify and monitor ITR sequence fidelity using Sanger sequencing and restriction enzyme digestion. Our special Sanger sequencing protocol guarantees a clear read-through of the ITR region, leaving no room for uncertainties. We also offer additional nanopore sequencing for quantitative ITR monitoring, further ensuring the utmost precision. Our rigorous processes, coupled with years of expertise in AAV packaging, make our AAV plasmids the most trusted choice for your gene therapy and research applications.

With PackGene, rest assured that you’ll receive top-quality plasmids that deliver exceptional results every time.

Learn more about PackGene’s AAV Packaging service

To produce high-quality mRNA, it is essential to begin with high-quality plasmid and linearization. Here at PackGene we ensure the highest quality linearized plasmid through several processes, including:

• Rigorous purification: We use a 3-step purification to remove impurities and endotoxins that are detrimental to in vitro mRNA production.
• Meticulous sequencing: We offer a 100% Gene of Interest (GOI) sequence guarantee as well as careful handling of poly A sequences.
• High-Yield Linearization: Our linearization process shows a nearly 100% linearization rate, which is the most important factor for efficient in vitro transcription of mRNA.
• Functional Verification: We verify plasmid quality by in-house mRNA production.