CRISPR/Cas9-rAAV

CRISPR is a state-of-the-art genetic modification tool that can be used to knock out, mutate, or overexpress a gene of interest. CRISPR-based research strategies have been widely employed across biological fields of study due to their powerful performance and relatively straightforward mechanism of action.

CRISPR gene editing requires the exogenous expression of the Cas9 enzyme and a 15-20nt guide RNA targeted to the experiment’s gene of interest. Recent advances have made the design, construction, and use of the CRISPR systems increasingly convenient. PackGene’s pre-made Cas9 vectors build on this convenience by providing packaged AAV vectors for Cas9 expression at lightning-fast turnaround times.

PackGene provides pre-made “off the shelf” CRISPR-AAV vectors that drive Cas9 expression under the EFS, miniCMV, or CMV broad-spectrum promoters as well as several tissue-specific promoters in the AAV9 serotype. Our in-stock pre-made products are shipped within one business day, making these products ideal for projects with a constrained timeline and that require standardized vectors.

PackGene provides high quality packaged AAVs for CRISPR Cas9 or CRISPR Cpf1 expression that can be directly applied in vivo and include a complete quality inspection report.