CRISPR gene editing requires the exogenous expression of the Cas9 enzyme and a 15-20nt guide RNA targeted to the experiment’s gene of interest. Recent advances have made the design, construction, and use of the CRISPR systems increasingly convenient. PackGene’s pre-made Cas9 vectors build on this convenience by providing packaged AAV vectors for Cas9 expression at lightning-fast turnaround times.
PackGene provides pre-made “off the shelf” CRISPR-AAV vectors that drive Cas9 expression under the EFS, miniCMV, or CMV broad-spectrum promoters as well as several tissue-specific promoters in the AAV9 serotype. Our in-stock pre-made products are shipped within one business day, making these products ideal for projects with a constrained timeline and that require standardized vectors.
PackGene provides high quality packaged AAVs for CRISPR Cas9 or CRISPR Cpf1 expression that can be directly applied in vivo and include a complete quality inspection report.
Promoter type | Catalog No. | Promoter and Gene |
Universal promoter |
AAV-XA20 |
ssAAV.miniCMV.SpCas9 |
AAV-XA02 |
ssAAV.EFS.SpCas9 |
|
AAV-XB02 |
ssAAV.EFS.SpCas9HF |
|
AAV-XB06 |
ssAAV.miniCMV.SpCas9HF |
|
AAV-AC010 |
ssAAV.EFS.SaCas9 |
|
AAV-AC040 |
ssAAV.CMV.SaCas9 |
|
AAV-AD040 |
ssAAV.CMV.SaCas9HF |
|
AAV-AD010 |
ssAAV.EFS.SaCas9HF |
|
AAV-XE01G |
ssAAV.EFS.AsCpfl |
|
AAV-XE01H |
ssAAV.EFS.LbCpfl |
|
AAV-XE02H |
ssAAV.miniCMV.LbCpfl |
|
Tissue-specific promotor |
AAV-AC050 |
ssAAV.TBG.SaCas9 |
AAV-AC060 |
ssAAV.Elastasel.SaCas9 |
|
AAV-AC070 |
ssAAV.Rat Insulin 2.SaCas9 |
|
AAV-AC080 |
ssAAV.mouse Insulin2.SaCas9 |
|
AAV-AC090 |
ssAAV.hDesmin.SaCas9 |
|
AAV-AC100 |
ssAAV.hSynapsin.SaCas9 |
|
AAV-AC120 |
ssAAV.pMECP2.SaCas9 |
|
AAV-AC190 |
ssAAV.CBh.Sacas9.WPRE.SV40pA |
|
AAV-AC071 |
ssAAV.Rat Insulin 2.SaCas9.U6.(Sa)sgRNA |
|
AAV-AC081 |
ssAAV.mouse Insulin2.SaCas9.U6.(Sa)sgRNA |
|
AAV-AC091 |
ssAAV.hDesmin.SaCas9.U6.(Sa)sgRNA |
|
AAV-AC101 |
ssAAV.hSynapsin.SaCas9.U6.(Sa)sgRNA | |
AAV-AC121 | ssAAV.pMECP2.SaCas9.U6.(Sa)sgRNA | |
AAV-AD060 | ssAAV.Elastasel.SaCas9HF | |
AAV-AD070 | ssAAV.rlnsulin2.SaCas9HF | |
AAV-AD080 | ssAAV.mlnsulin2.SaCas9HF | |
AAV-AD090 | ssAAV.hDesmin.SaCas9HF | |
AAV-AD100 | ssAAV.hSynapsin.SaCas9HF | |
AAV-AD120 | ssAAV.MECP2.SaCas9HF |
(Scroll down to see more)
![AAV AAV](https://www.packgene.com/wp-content/uploads/2023/02/3-1.png)
Injection Method: Intramuscular Injection
Recommended Dose: 25 μL, 1.5E+12 vg/ml
Targeting Site: muscle
Animal Model: DMD mouse model
Journal: Genome Medicine, 2021 (IF=15.266)
Paper Title: In vivo genome editing in mouse restores dystrophin expression in Duchenne muscular dystrophy patient muscle fibers
DOI: 10.1186/s13073-021-00876-0
![](https://www.packgene.com/wp-content/uploads/2023/02/3-2.jpg)
CRISPR AAV vectors were generated by PackGene Biotech Co. For intramuscular injection into NSI mice, the animals were anesthetized by abdominal injection of 5% chloral hydrate, followed by
injection of CRISPR AAV vector (25 μL, 1.5E+12 vg) into the TA muscle transplanted with DMD–MDSCs.
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